Journal: Immunity, Inflammation and Disease

Article Title: Didymin may Enhance Tendon‐Bone Healing Partly by Promoting PPAR‐Mediated Fatty Acid Oxidation and Macrophage M2 Polarization

doi: 10.1002/iid3.70485

Figure Lengend Snippet: Effect of DID on macrophage polarization in tendon‐bone healing mice. (A) Representative images from flow cytometric analysis of F4/80 + CD86 + ; (B) quantitative analysis of F4/80 + CD86 + ; (C) representative images from flow cytometric analysis of F4/80 + CD206 + ; (D) quantitative analysis of F4/80 + CD206 + ; (E) levels of IL‐1β mRNA as measured by qRT‐PCR; (F) levels of IL‐6 mRNA as measured by qRT‐PCR; (G) levels of Arg1 mRNA as measured by qRT‐PCR; (H) levels of IL‐10 mRNA as measured by qRT‐PCR. Data are expressed as the mean ± SD ( n = 3 biological replicates). * p < 0.05, ** p < 0.01 vs. sham group; # p < 0.05, ## p < 0.01 vs. model group.

Article Snippet: The Sox9 (Cat No., BS‐10725R), Collagen II (Cat No., bsm‐33409M), F4/80 (Cat No. 263101), CD86 (Cat No. bs‐1035R), and CD206 (Cat No. bsm‐60761R) antibodies were bought from Bioss (China).

Techniques: Analysis, Quantitative RT-PCR

Journal: Immunity, Inflammation and Disease

Article Title: Didymin may Enhance Tendon‐Bone Healing Partly by Promoting PPAR‐Mediated Fatty Acid Oxidation and Macrophage M2 Polarization

doi: 10.1002/iid3.70485

Figure Lengend Snippet: Effect of DID on macrophage polarization and PPAR signaling‐mediated FAO pathway in BMDMs. (A) Representative pictures of immunofluorescence staining (20×, 50 μm) of F4/80 + CD86 + and F4/80 + CD206 + in response to LPS/IFN‐γ co‐stimulation; (B) quantitative analysis of F4/80 + CD86 + and F4/80 + CD206 + ; (C) representative pictures of immunofluorescence staining (20×, 50 μm) of F4/80 + CD86 + and F4/80 + CD206 + in response to IL‐4/IL‐13 co‐stimulation; (D) quantitative analysis of F4/80 + CD86 + and F4/80 + CD206 + ; (E) levels of CoA were detected by ELISA; (F) levels of Cpt1a mRNA as measured by qRT‐PCR; (G) levels of ACS mRNA as measured by qRT‐PCR; (H) levels of PPAR‐γ protein as measured by Western blotting; (I) levels of RXRA protein as measured by Western blotting; (J) protein bands were analyzed by Western blotting for PPAR‐γ and RXRA. Data are expressed as the mean ± SD ( n = 3 biological replicates). * p < 0.05, ** p < 0.01 vs. BMDMs (M0) group; # p < 0.05, ## p < 0.01 vs. BMDMs (M1) group.

Article Snippet: The Sox9 (Cat No., BS‐10725R), Collagen II (Cat No., bsm‐33409M), F4/80 (Cat No. 263101), CD86 (Cat No. bs‐1035R), and CD206 (Cat No. bsm‐60761R) antibodies were bought from Bioss (China).

Techniques: Immunofluorescence, Staining, Analysis, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, Western Blot

Journal: Immunity, Inflammation and Disease

Article Title: Didymin may Enhance Tendon‐Bone Healing Partly by Promoting PPAR‐Mediated Fatty Acid Oxidation and Macrophage M2 Polarization

doi: 10.1002/iid3.70485

Figure Lengend Snippet: Validation of PPAR signaling‐mediated FAO pathway involved in DID‐induced macrophage polarization in BMDMs. (A) Levels of PPAR‐γ and RXRA protein as measured by Western blotting; (B) levels of CoA were detected by ELISA; (C) immunofluorescence staining (20×, 50 μm) of F4/80 + CD86 + and F4/80 + CD206 + ; (D) levels of Arg1, IL‐1β, IL‐10, and IL‐6 mRNA as measured by qRT‐PCR. Data are expressed as the mean ± SD ( n = 3 biological replicates). * p < 0.05, ** p < 0.01 vs. BMDMs (M0) group; # p < 0.05, ## p < 0.01 vs. BMDMs (M1) group; & p < 0.05, && p < 0.01 vs. BMDMs (M1) + DID group.

Article Snippet: The Sox9 (Cat No., BS‐10725R), Collagen II (Cat No., bsm‐33409M), F4/80 (Cat No. 263101), CD86 (Cat No. bs‐1035R), and CD206 (Cat No. bsm‐60761R) antibodies were bought from Bioss (China).

Techniques: Biomarker Discovery, Western Blot, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Staining, Quantitative RT-PCR

Journal: Stem Cells International

Article Title: CD73‐Overexpressing ADSCs Promote Neurogenic Bladder Repair via A2A–JAK1/STAT3‐Mediated Modulation of Inflammation and Macrophage Polarization

doi: 10.1155/sci/8564160

Figure Lengend Snippet: CD73‐overexpressing ADSCs regulate macrophage polarization toward the M2 and inhibit NLRP3 inflammasome activation. (A) Flow cytometry results showing macrophage polarization in each group. CD86 was detected using FITC‐conjugated antibody, and CD163 was detected using PE‐conjugated antibody. (B) Western blot and quantitative analysis of NLRP3, ASC, and caspase‐1 expression in vitro. (C) Quantitative analysis of Western blot. (D) ELISA analysis of IL‐1β and IL‐18 levels in each group in vitro. Statistical significance is indicated as ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001. All experiments were independently performed in triplicate. The full‐length, uncropped Western blot images are provided in Figure . ELISA data are summarized in Tables and .

Article Snippet: For intracellular marker staining, cells were blocked with 5% bovine serum albumin and incubated for 30 min at room temperature with FITC‐conjugated antimouse CD86 (Elabscience, Wuhan, Hubei, China; 1:100 dilution) and PE‐conjugated antimouse CD163 antibodies (Elabscience, Wuhan, Hubei, China; 1:100 dilution).

Techniques: Activation Assay, Flow Cytometry, Western Blot, Analysis, Expressing, In Vitro, Enzyme-linked Immunosorbent Assay